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| Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de. |
| Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020 |
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| Kumar,Namrata; Moreno,Natália C.; Feltes,Bruno C.; Menck,Carlos FM; Houten,Bennett Van. |
| Abstract Base and nucleotide excision repair (BER and NER) pathways are normally associated with removal of specific types of DNA damage: small base modifications (such as those induced by DNA oxidation) and bulky DNA lesions (such as those induced by ultraviolet or chemical carcinogens), respectively. However, growing evidence indicates that this scenario is much more complex and these pathways exchange proteins and cooperate with each other in the repair of specific lesions. In this review, we highlight studies discussing the involvement of NER in the repair of DNA damage induced by oxidative stress, and BER participating in the removal of bulky adducts on DNA. Adding to this complexity, UVA light experiments revealed that oxidative stress also causes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Base excision repair; Nucleotide excision repair; DNA damage; Protein oxidation; UVA light. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200309 |
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